Chromatography refers to a group of mixture separation techniques, which are widely applied in fields including analytical chemistry, organic chemistry and biochemistry.
By utilizing the differences in physical and chemical properties of components in a mixture, chromatography realizes the separation and determination of multi-component mixtures.
Based on chromatographic mechanisms, it can be divided into adsorption chromatography, partition chromatography, ion-exchange chromatography, gel filtration chromatography and so on.
Specific applications of chromatography include:
Desalination: for instance, using gel chromatography to remove low-molecular-weight impurities from solutions of macromolecules (such as proteins, nucleic acids, polysaccharides, etc.);
Separation and purification: used for the separation and purification of enzymes, proteins, polysaccharides, hormones, alkaloids and other substances;
Concentration of dilute solutions.
Industry Overview
The specific applications of chromatography include
Desalination: for example, using gel chromatography to remove low-molecular-weight impurities from solutions of macromolecules such as proteins, nucleic acids and polysaccharides;
Separation and purification: applied to the separation and purification of enzymes, proteins, polysaccharides, hormones, alkaloids and other substances;
Concentration of dilute solutions;
Molecular weight determination of macromolecular substances;
Research on various chromatographic methods and the performance of chromatographic packings, among other applications.
customer demand
solution
The mini peristaltic pump CM200-1A is mainly designed for laboratory use.Its speed can be adjusted manually or automatically via an external control interface.It is matched with the BPH01 pump head and compatible with various tubing sizes: No.13, No.14, No.19, No.16 and No.25.The maximum reference flow rate is 170 mL/min.
As shown in the diagram, the peristaltic pump is connected upstream of the chromatography column, continuously pumping the mobile phase, eluent and other solutions into the column.
The eluted solution is collected into separate containers according to its different components.
Application Value
Peristaltic pumps provide stable fluid pressure, enabling smooth passage through chromatography columns with high resistance (smaller packing pores result in greater resistance).
Featuring high precision in fluid delivery, they ensure consistent flow rates through the column to achieve the desired chromatographic performance.
With low shear force, they minimize damage to active ingredients when pumping macromolecular solutions such as proteins.
Hose replacement is quick and easy. In laboratories, cross-contamination between different solutions can be avoided simply by replacing hygienic-grade tubing.
Compact in structure, they support stacked installation, greatly saving valuable laboratory bench space and enabling flexible layout with maximized space utilization.
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